lipolytica is one of the most divergent of the characterized Hemiascomycetes. The oleaginous yeast Yarrowia lipolytica is an industrial model organism for production of biosustainable hydrocarbon-based chemicals. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. Van Etten, PI) at the University of California, Irvine and NIH shared instrumentation grants 1S10RR025496-01 and 1S10OD010794-01 to SS. This work was made possible, in part, through access to the Genomic High Throughput Facility Shared Resource of the National Cancer Institute Cancer Center Support Grant (CA-62203, R. EEC-0813570, B.Shanks, PI subcontract SS) through the Engineering Research Center for Biorenewable Chemicals, CBiRC), and National Institutes of Health LM010235, NIH-National Library of Medicine 07443, and NSF IIS-1321053 to PB. It will in the future also be available at the UCI Institute for Genomics and Bioinformatics server ( ).įunding: This research was supported by the National Science Foundation (Grant no. We have also made the sequence available in annotated form on our own server at. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: Sequence data and annotations are available from the National Center for Biotechnology Information (NCBI) Bioproject PRJNA295780/BioSample SAMN04088558 ( ). Received: Accepted: AugPublished: September 7, 2016Ĭopyright: © 2016 Magnan et al. PLoS ONE 11(9):Įditor: Joseph Schacherer, University of Strasbourg, FRANCE (2016) Sequence Assembly of Yarrowia lipolytica Strain W29/CLIB89 Shows Transposable Element Diversity. Differences between the sequence of this strain and of the existing reference strain underscore the utility of an additional independent genome assembly for this economically important organism.Ĭitation: Magnan C, Yu J, Chang I, Jahn E, Kanomata Y, Wu J, et al. lipolytica genome, they could have played an active role in its evolution. Therefore, although identified transposons do not constitute a significant fraction of the Y. Multiple full-length and truncated LINE elements are also present. In addition to previously-reported dimeric RNA polymerase III-transcribed genes, tRNA pseudogenes were identified. lipolytica compared to other Hemiascomycetes. Strikingly, several of these are adjacent to RNA polymerase III-transcribed genes, which are almost double in number in Y. Two large structural variants and retroelement differences with reference strain CLIB122 including a full-length, novel T圓/Gypsy long terminal repeat (LTR) retrotransposon and multiple LTR-like sequences are described. The telomeric rDNA repeats were localized by Irys long-range genome mapping and one complete copy of the rDNA sequence is reported. lipolytica chromosome is represented by a single contig. We report here a de novo annotated assembly of the chromosomal genome of an industrially-relevant strain, W29/CLIB89, determined by hybrid next-generation sequencing. Despite its economic importance and several distinct strains in common use, an independent genome assembly exists for only one strain. lipolytica genome is almost double that of model yeasts. In spite of a similar number of protein-coding genes to that in other Hemiascomycetes, the Y. Yarrowia lipolytica, an oleaginous yeast, is capable of accumulating significant cellular mass in lipid making it an important source of biosustainable hydrocarbon-based chemicals.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |